Key Takeaway
Getting the BPC-157 TB-500 dosing schedule right can make a significant difference in your results. These two peptides are often combined in what is known as the Wolverine Stack, a popular protocol for tissue repair and recovery. But the way you structure your doses, timing, and phases matters.
Getting the BPC-157 TB-500 dosing schedule right can make a significant difference in your results. These two peptides are often combined in what is known as the Wolverine Stack, a popular protocol for tissue repair and recovery. But the way you structure your doses, timing, and phases matters. In this guide, we break down loading versus maintenance phases, daily versus weekly dosing, and practical tips for organizing your schedule.
Key Takeaways: - Understanding Loading and Maintenance Phases - Sample Weekly Dosing Schedules - Injection Site Rotation and Best Practices - When to Adjust Your Schedule
How Loading and Maintenance Phases
Most BPC-157 and TB-500 protocols involve two distinct phases. The loading phase uses higher doses to jumpstart the healing process. The maintenance phase uses lower doses to support ongoing repair.
Loading phase (weeks 1-2): During this period, your provider may prescribe higher doses to build up peptide levels in your system. For TB-500, loading doses typically range from 750 mcg administered 2-3 times per week. BPC-157 loading doses usually range from 500 mcg once or twice daily.
Maintenance phase (weeks 3-8): After the initial loading period, doses are typically reduced. TB-500 maintenance is often 750 mcg once per week. BPC-157 may stay at 250-500 mcg once daily or be reduced to 250 mcg.
The logic behind this approach is that TB-500 has a longer half-life and builds up in your system. Once you have established adequate levels, you need less frequent dosing to maintain them. BPC-157 has a shorter half-life and generally requires more consistent daily dosing throughout the protocol.
Your provider may adjust this structure based on your response. If you are healing faster than expected, they might shorten the loading phase. If progress is slow, they might extend it. This is why regular check-ins during your protocol are important.
For a detailed overview of how these peptides work together, read .
"The key to successful GLP-1 therapy is setting realistic expectations and supporting patients through the titration phase. The side effects are manageable for most people, but they need to know what to expect.") Dr. Caroline Apovian, MD, Harvard Medical School
Sample Weekly Dosing Schedules
Here are two common weekly schedules that providers use. Remember that your provider will customize your specific protocol.
Standard Protocol:
| Day | BPC-157 | TB-500 |
|---|---|---|
| Monday | 250 mcg AM, 250 mcg PM | 750 mcg |
| Tuesday | 250 mcg AM, 250 mcg PM | , |
| Wednesday | 250 mcg AM, 250 mcg PM | , |
| Thursday | 250 mcg AM, 250 mcg PM | 750 mcg |
| Friday | 250 mcg AM, 250 mcg PM | , |
| Saturday | 250 mcg AM, 250 mcg PM | , |
| Sunday | Rest | Rest |
Simplified Protocol:
| Day | BPC-157 | TB-500 |
|---|---|---|
| Daily | 500 mcg once daily | , |
| Monday & Thursday | , | 750 mcg |
The simplified protocol works well for people who want fewer injections. The standard protocol may offer more consistent peptide levels throughout the day due to BPC-157's short half-life.
Injection timing also matters. Many providers recommend injecting BPC-157 as close to the injury site as possible. TB-500 can be injected subcutaneously in the abdomen since it works systemically.
For accurate dose preparation every time, .
Free Download: Wolverine Stack Protocol Card A printable dosing schedule card with loading and maintenance phases, injection site guidance, and weekly tracking checkboxes. Get yours free (we'll email it to you instantly. [Download Now]
Injection Site Rotation and Best Practices
Proper injection technique keeps your protocol safe and effective. Here are the practical details that matter.
Check your GLP-1 eligibility
Use our free BMI Calculator to see if you may qualify for physician-supervised GLP-1 therapy.
Try the BMI Calculator →Injection sites for BPC-157: Inject subcutaneously as close to the area you are treating as possible. For a shoulder injury, inject in the deltoid area. For a knee issue, inject near the knee. For gut-related protocols, inject in the lower abdomen. Rotate between 2-3 sites near the treatment area to prevent irritation.
Injection sites for TB-500: Since TB-500 works systemically, it does not need to be injected near the injury. The abdomen is the most common site. Rotate between left and right sides, staying at least 2 inches from your belly button.
General injection tips: Use an alcohol swab to clean the injection site. Pinch the skin and insert the needle at a 45-degree angle. Inject slowly. After removing the needle, apply gentle pressure with a clean swab. Do not rub the site.
Storage matters: Reconstituted peptides should be stored in the refrigerator and used within the timeframe your pharmacy specifies, typically 28-30 days. Do not freeze reconstituted peptides. Keep the vials upright and handle them gently to prevent degradation.
Track each injection in a log noting the date, time, site, dose, and any reactions. This helps you maintain proper rotation and gives your provider useful data. The has built-in injection tracking for exactly this purpose.
When to Adjust Your Schedule
Your initial dosing schedule is a starting point, not a fixed plan. Here are signs that your provider may need to adjust your protocol.
Signs you may need a higher dose or longer loading phase: No improvement after 2-3 weeks, persistent inflammation that is not responding, or a more severe injury than initially assessed.
Signs your dose may be too high: Excessive fatigue, headaches, digestive upset, or significant injection site reactions that do not improve. These symptoms do not always mean the dose is too high, but they warrant a conversation with your provider.
Signs you are ready to transition to maintenance: Noticeable reduction in pain, improved range of motion, decreased swelling, and consistent daily improvement. Most people transition after 2 weeks, but your provider will make the call based on your response.
Signs you may be ready to end the cycle: Significant functional improvement, pain levels at 2 or below on a 10-point scale, and stable progress for at least 1-2 weeks. Your provider will confirm when it is appropriate to stop.
Never adjust your dosing schedule on your own. Even small changes to peptide doses or timing can affect your results. Always consult your provider before making changes.
For more context on peptide cycling and when to take breaks, explore .
Frequently Asked Questions
Can I take BPC-157 and TB-500 in the same syringe?
Some providers allow combining both peptides in one syringe for convenience. Others prefer separate injections. Follow your provider's specific instructions. If combining, ensure both peptides are reconstituted with compatible solutions.
What time of day is best for injections?
For BPC-157, morning and evening dosing is common when using a twice-daily protocol. For TB-500, time of day is less critical since it has a longer half-life. Many people choose morning for convenience and consistency.
What if I miss a dose?
If you miss a BPC-157 dose, take it as soon as you remember unless it is close to your next scheduled dose. Do not double up. If you miss a TB-500 dose, take it as soon as you remember or on your next scheduled day. Missing an occasional dose is unlikely to significantly impact your results.
How long should I wait between cycles?
Most providers recommend 2-4 weeks off between cycles. This allows your receptors to reset and gives your body time to handle healing independently. Your provider will determine the right off-cycle duration based on your progress.
Do I need blood work during this protocol?
Most providers recommend baseline blood work before starting and a follow-up panel mid-cycle or after completion. This is especially important if you are stacking with growth hormone peptides. For a standalone Wolverine Stack, blood work requirements may be minimal.
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Sources & References
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- Chang CH, Tsai WC, Lin MS, et al. The promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migration. J Appl Physiol. 2011;110(3):774-780. Doi:10.1152/japplphysiol.00945.2010
- Seiwerth S, Brcic L, Vuletic LB, et al. BPC 157 and blood vessels. Curr Pharm Des. 2014;20(7):1121-1125. Doi:10.2174/13816128113199990421
- Bock-Marquette I, Saxena A, White MD, et al. Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair. Nature. 2004;432(7016):466-472. Doi:10.1038/nature03000
- Malinda KM, Sidhu GS, Mani H, et al. Thymosin beta4 accelerates wound healing. J Invest Dermatol. 1999;113(3):364-368. Doi:10.1046/j.1523-1747.1999.00708.x
- Ionescu M, Frohman LA. Pulsatile secretion of growth hormone (GH) persists during continuous stimulation by CJC-1295, a long-acting GH-releasing hormone analog. J Clin Endocrinol Metab. 2006;91(12):4792-4797. Doi:10.1210/jc.2006-1702
This content is provided for informational and educational purposes only. It is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the advice of a licensed healthcare provider with any questions about a medical condition or treatment plan.
Last updated: 2026-03-24