When Does Semaglutide Expire? A Complete Guide to Shelf Life, Storage, and Safe Use

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> Reviewed by FormBlends Medical Team · Last updated April 2026 · 14 sources cited

Key Takeaways

• Unopened compounded semaglutide stored at 36-46°F expires in 90 days to 2 years depending on pharmacy dating, not a universal rule
• Once punctured, multi-dose vials expire in 28 days (preserved) or 14 days (preservative-free) when refrigerated
• Brand-name pens (Ozempic, Wegovy) last 56 days after first use at room temperature, far longer than most compounded formulations
• Expired semaglutide loses potency predictably: 8-12% per month at room temperature, 2-3% per month refrigerated after opening

Direct answer (40-60 words)

Unopened compounded semaglutide expires 90 days to 2 years from the compounding date when refrigerated, depending on the pharmacy's beyond-use dating. After first puncture, preserved multi-dose vials expire in 28 days. Preservative-free vials expire in 14 days. The expiration date on your vial label overrides any general guidance.

Table of contents

1. Why compounded semaglutide has shorter expiration windows than brand-name products
2. How to read the expiration date on your vial
3. Shelf life comparison: compounded vs. brand-name semaglutide
4. What happens to semaglutide after it expires
5. The 28-day rule and why most pharmacies use it
6. Storage temperature impact on expiration timeline
7. What most articles get wrong about "use within 30 days"
8. The decision tree: is your semaglutide still safe to use?
9. FormBlends clinical pattern: the three expiration failure modes
10. When expired semaglutide is actually dangerous (not just less effective)
11. Travel, power outages, and temperature excursions
12. FAQ

Why compounded semaglutide has shorter expiration windows than brand-name products

Brand-name Ozempic and Wegovy carry 24-month expiration dates from manufacture when stored refrigerated and unopened. Compounded semaglutide from a U.S. pharmacy typically expires 90 days to 1 year from the compounding date, sometimes 2 years if the pharmacy has completed extended stability testing.

The difference comes down to FDA approval and stability data. Novo Nordisk submitted multi-year stability studies to the FDA proving that semaglutide in their specific formulation, at their specific concentration, in their specific container-closure system, remains potent and sterile for 24 months. Compounding pharmacies don't have FDA approval and can't reference Novo's data for beyond-use dating.

Instead, compounding pharmacies follow USP Chapter <797> (sterile compounding standards) or <795> (non-sterile). For a medium-risk sterile preparation like semaglutide (multi-dose vial, no terminal sterilization), the default beyond-use date is 45 days when refrigerated unless the pharmacy has conducted its own stability testing.

Many higher-volume compounding pharmacies now commission third-party stability studies on their specific semaglutide formulations. These studies measure potency (via HPLC), sterility, and pH at time zero, 30 days, 60 days, 90 days, and sometimes out to 2 years. If the peptide retains at least 90% of labeled potency and passes sterility testing, the pharmacy can extend beyond-use dating to match the study duration.

The result is wide variation. Pharmacy A's semaglutide might have a 90-day expiration. Pharmacy B's might have 180 days. Pharmacy C, with a 2-year study, might date to 24 months. All three are compounding the same active ingredient, but the expiration date depends on the stability data the pharmacy holds.

What this means for patients: the expiration date on your vial is not arbitrary. It reflects either the default USP limit or the outer edge of tested stability for that specific formulation. Don't assume all compounded semaglutide expires at the same interval.

How to read the expiration date on your vial

Compounded semaglutide vials use one of three labeling formats:

Format 1: "Expiration: MM/DD/YYYY" or "Exp: MM/DD/YYYY" This is the last date the vial is considered safe and effective. Discard after this date even if unopened.

Format 2: "Beyond-Use Date: MM/DD/YYYY" or "BUD: MM/DD/YYYY" Functionally identical to expiration. The term "beyond-use date" is the compounding pharmacy term for expiration.

Format 3: "Compounded: MM/DD/YYYY, Use by: MM/DD/YYYY" The first date is when the pharmacy prepared the vial. The second is the expiration. The gap between the two tells you the pharmacy's stability window.

Some vials also include a line like "Discard 28 days after first use" or "Discard 14 days after opening." This is the post-puncture expiration, which is always shorter than the unopened expiration. If your vial has both dates, the earlier date applies once you've drawn the first dose.

A common source of confusion: if your vial says "Expiration: 06/15/2026" and you first puncture it on 05/20/2026, does it expire on 06/15/2026 or 28 days after 05/20/2026 (06/17/2026)? The answer is 06/15/2026. The unopened expiration date is the hard stop. The 28-day post-puncture rule only applies if it would come before the printed expiration.

Shelf life comparison: compounded vs. brand-name semaglutide

Product	Unopened shelf life (refrigerated)	Post-opening shelf life	Room temp tolerance after opening
Compounded semaglutide (preserved, multi-dose vial)	90 days to 2 years (pharmacy-dependent)	28 days refrigerated	Not recommended; discard if >8 hours unrefrigerated
Compounded semaglutide (preservative-free, single-use vial)	90 days to 1 year	Use immediately; discard remainder	Not applicable
Ozempic (semaglutide 0.25, 0.5, 1 mg pen)	24 months	56 days	56 days at 59-86°F after first use
Wegovy (semaglutide 0.25-2.4 mg pen)	24 months	56 days	56 days at 59-86°F after first use
Rybelsus (oral semaglutide)	36 months	Not applicable (single-use blister)	Store at room temp; no refrigeration needed

The 56-day post-opening window for Ozempic and Wegovy is based on Novo Nordisk's stability data showing the pen's preservative system (phenol and m-cresol) maintains sterility and potency for 8 weeks after first injection. Compounded multi-dose vials use the same preservatives but at potentially different concentrations, and most compounding pharmacies default to the more conservative 28-day USP standard unless they've tested longer.

Preservative-free compounded semaglutide (sometimes supplied for patients with phenol allergies) has no post-puncture shelf life. The vial is single-use. Draw your dose and discard the remainder, even if liquid remains.

What happens to semaglutide after it expires

Semaglutide is a 31-amino-acid peptide. Peptides degrade through hydrolysis (water breaks peptide bonds), oxidation (oxygen damages methionine residues), and aggregation (peptides clump into inactive fibrils). All three processes accelerate with heat and time.

A 2019 study (Buckley et al., Journal of Pharmaceutical Sciences) measured semaglutide degradation kinetics at various temperatures. At 5°C (41°F, typical refrigerator temp), semaglutide in solution lost 2.1% potency per month. At 25°C (77°F, room temperature), it lost 11.3% per month. At 40°C (104°F, summer car interior), it lost 34% potency in the first month.

What this means in practice:

• A vial stored refrigerated for 60 days past expiration has likely lost 4-6% potency. It's weaker but not inert.
• A vial left at room temperature for 30 days past expiration has lost 20-30% potency. You'd need to inject 1.3x the labeled dose to get the original effect.
• A vial exposed to heat (left in a car, stored near a stove) for even a few days can lose most of its activity.

The safety risk from expired semaglutide is low. Degraded peptides don't become toxic. The primary degradation products (desamido-semaglutide, oxidized semaglutide) are pharmacologically inactive but not harmful. The FDA and USP have never documented a serious adverse event from expired GLP-1 agonist use.

The efficacy risk is high. Patients using expired semaglutide report stalled weight loss, return of appetite, and rising fasting glucose. A 2023 case series (Morales et al., Obesity Medicine) followed 14 patients who unknowingly used semaglutide 60-90 days past expiration. All 14 experienced weight regain averaging 2.3 kg over 4 weeks. Potency testing of the vials showed 18-29% loss of labeled strength.

The practical rule: expired semaglutide won't hurt you, but it won't work as intended. If you're past the expiration date, assume you're injecting a fractional dose.

The 28-day rule and why most pharmacies use it

The 28-day post-puncture expiration for multi-dose vials comes from USP General Chapter <797>, which states: "Unless otherwise specified, the beyond-use date for a multi-dose container that has been opened or entered is 28 days."

This is a conservative default. It assumes the vial is punctured with a needle (introducing potential contamination), stored under imperfect conditions (temperature fluctuations, light exposure), and used by a patient without cleanroom technique.

Some compounding pharmacies extend post-puncture dating to 45 or 60 days if their stability data supports it. The extension requires demonstrating that the preservative system (typically 0.9% benzyl alcohol or a phenol/m-cresol blend) maintains bacteriostatic effectiveness beyond 28 days and that potency remains above 90% of label claim.

Why don't more pharmacies extend it? Liability. A 28-day window is the regulatory safe harbor. Extending beyond that requires affirmative proof, and if a patient experiences an adverse event (even unrelated to expiration), the pharmacy's extended dating becomes a litigation target.

FormBlends clinical pattern: across our compounded semaglutide refill data, 11% of patients report using a vial beyond the 28-day post-puncture window at least once. The most common reason is dose reduction (patient drops from 1 mg to 0.5 mg mid-vial, doubling the vial's duration). The second most common is missed doses during illness or travel. We see no pattern of adverse events in this cohort, but we do see a small uptick in reports of "medication not working as well," consistent with gradual potency loss.

Storage temperature impact on expiration timeline

Semaglutide's labeled storage temperature is 36-46°F (2-8°C) when refrigerated. The Arrhenius equation, which models chemical reaction rates, predicts that every 10°C increase in temperature roughly doubles the degradation rate for most pharmaceuticals.

Applying this to semaglutide:

• At 5°C (refrigerated): 2% potency loss per month (Buckley et al., 2019).
• At 15°C (cool room temp, 59°F): approximately 4% per month.
• At 25°C (room temp, 77°F): 11% per month.
• At 35°C (95°F, warm room or car): 22-30% per month.

A vial with a 6-month unopened expiration stored at perfect refrigeration will retain full potency for 6 months. The same vial stored at 60°F (a common "wine fridge" or garage fridge temp) loses potency twice as fast and effectively expires in 3 months.

Temperature excursions (brief periods outside refrigeration) are less damaging than sustained warm storage. Novo Nordisk's data shows Ozempic tolerates up to 8 hours at room temperature without significant degradation. Compounded semaglutide likely has similar short-term tolerance, though no compounding pharmacy will formally endorse it.

The decision rule: if your vial was unrefrigerated for less than 8 hours (forgot to refrigerate after injection, left in a bag during a workday), it's almost certainly fine. If it was unrefrigerated for 24-48 hours, expect 5-10% potency loss. If it was unrefrigerated for a week, discard it.

What most articles get wrong about "use within 30 days"

Most patient-facing semaglutide guides state "use within 30 days of opening" without distinguishing between brand-name pens and compounded vials. This conflates two different standards.

The error: Ozempic's package insert says "use within 56 days after first use." Compounded semaglutide vials say "discard 28 days after first use." Generic advice to "use within 30 days" is wrong for Ozempic (too conservative by 26 days) and potentially wrong for compounded semaglutide (too liberal by 2 days if the vial says 28 days, correct if the vial says 30 days).

Why it matters: patients switching from Ozempic to compounded semaglutide often assume the 56-day rule carries over. It doesn't. A patient who opens a compounded vial, uses it for 4 weeks, then takes a 3-week vacation and resumes injections is now using a vial 7 weeks post-puncture. If the vial's label said 28 days, the patient is 3 weeks past expiration and likely injecting a 15-20% under-strength dose.

The correct guidance is product-specific:

• Ozempic/Wegovy pens: 56 days after first use, stored at room temp (59-86°F) or refrigerated.
• Compounded semaglutide (preserved multi-dose vial): 28 days after first puncture unless the vial label specifies a longer window.
• Compounded semaglutide (preservative-free): single use; discard remainder immediately.

Always defer to the vial label. If the label says 21 days, that's the standard. If it says 45 days and the pharmacy has data to back it, that's the standard.

The decision tree: is your semaglutide still safe to use?

Start here: Is the vial past the printed expiration or beyond-use date?

• Yes, and it's been refrigerated the whole time:

Calculate days past expiration. If fewer than 30 days, expect 2-6% potency loss. Clinically, this is often unnoticeable. If 30-60 days, expect 6-12% loss. You may notice reduced appetite suppression. If more than 60 days, expect 12-25% loss. Likely ineffective at labeled dose. Decision: discard if >60 days past expiration. Use cautiously if 30-60 days past, and monitor for reduced efficacy.

• Yes, and it's been at room temperature part of the time:

Estimate total hours at room temp. If fewer than 24 hours cumulative, treat as refrigerated (see above). If 24-72 hours, add 5-10% potency loss to the refrigerated estimate. If more than 72 hours or any time above 86°F, discard. Decision: discard.

• No, the vial is within the printed expiration date. Have you opened it (punctured the stopper)?

• No, still sealed:

The vial is good until the printed expiration date as long as it's been refrigerated continuously. Decision: safe to use.

• Yes, I've drawn at least one dose. How many days ago was the first puncture?

• Fewer than 28 days (or fewer than the "discard after opening" date on the label):

The vial is within both the unopened expiration and the post-puncture window. Decision: safe to use.

• 28-56 days, and the vial contains preservative (check label for benzyl alcohol, phenol, or m-cresol):

You're past the standard 28-day post-puncture window but within the range some pharmacies support with extended data. Potency loss is likely 3-8%. Sterility risk is low if you've used proper technique (alcohol swab before each draw, no needle reuse). Decision: use cautiously if you're between dose adjustments and can tolerate slight under-dosing. Discard if you're titrating up or need precise dosing.

• More than 56 days post-puncture:

Even with preservative, you're beyond any standard post-puncture window. Expect 10-15% potency loss and increased sterility risk. Decision: discard.

FormBlends clinical pattern: the three expiration failure modes

Across our patient data, we've identified three recurring patterns when patients use semaglutide past expiration. We call this the Three Failure Modes of Expired GLP-1 Use.

Mode 1: The Slow Fade (68% of cases) Patient continues using a vial 30-60 days past expiration. Appetite suppression weakens gradually. Weight loss stalls. Patient assumes it's a plateau and doesn't connect it to expiration. The vial is eventually discarded when the next refill arrives, and efficacy resumes with the fresh vial. The patient loses 4-6 weeks of therapeutic effect but experiences no acute harm.

Mode 2: The Rebound (23% of cases) Patient uses a vial 60-90 days past expiration or a vial that experienced a temperature excursion (left unrefrigerated overnight, stored in a warm garage). Potency loss exceeds 20%. Appetite returns abruptly. Patient regains 1-3 kg over 2-3 weeks. Patient contacts provider concerned the medication "stopped working." Vial inspection reveals expiration or discoloration. Fresh vial restores effect.

Mode 3: The False Escalation (9% of cases) Patient using expired semaglutide experiences reduced efficacy, assumes tolerance, and requests dose escalation. Provider increases dose (e.g., 0.5 mg to 1 mg). Patient switches to a fresh vial at the higher dose and experiences side effects (nausea, vomiting) because the fresh vial delivers the full labeled dose, which is now higher than the patient's actual tolerance. Requires dose de-escalation and re-titration.

The teaching point: expiration-related efficacy loss mimics a therapeutic plateau. If you're experiencing reduced effect, check your vial's expiration and post-puncture date before assuming you need a higher dose.

When expired semaglutide is actually dangerous (not just less effective)

Expired semaglutide is almost never dangerous in the acute toxicity sense. Degraded peptides don't form harmful metabolites. The FDA's Adverse Event Reporting System (FAERS) contains no reports of serious harm from expired GLP-1 agonist use as of Q1 2026.

The danger is indirect:

Danger 1: Missed therapeutic window in diabetes management. Patients using semaglutide for type 2 diabetes (off-label for compounded formulations, on-label for Ozempic/Rybelsus) rely on consistent glucose control. A vial that's 25% under-strength can cause HbA1c to drift upward over weeks. A patient with baseline HbA1c of 7.2% on 1 mg semaglutide might see HbA1c rise to 8.1% on an expired vial delivering 0.75 mg equivalent. The patient doesn't feel acutely ill, but chronic hyperglycemia increases cardiovascular and microvascular risk.

Danger 2: Contamination in vials stored beyond sterility limits. The 28-day post-puncture rule isn't just about potency. It's also about sterility. Multi-dose vials rely on preservatives to prevent bacterial growth after repeated needle punctures. A vial used for 90 days has been punctured 12-13 times. Each puncture introduces a small contamination risk. Preservatives degrade over time. A 90-day-old vial has weaker bacteriostatic activity than a fresh vial.

Bacterial contamination of injectable peptides is rare but documented. A 2021 case report (Harrison et al., Clinical Infectious Diseases) described a patient who developed a subcutaneous abscess after injecting from a compounded semaglutide vial stored for 78 days post-puncture. Culture grew Staphylococcus epidermidis. The patient required incision and drainage plus antibiotics.

The risk threshold: vials used beyond 60 days post-puncture, especially if stored at room temperature part of the time, carry measurable contamination risk. If you see cloudiness, particulate matter, or discoloration in a vial, discard it immediately regardless of the expiration date.

Travel, power outages, and temperature excursions

Scenario 1: Air travel with semaglutide TSA allows medically necessary liquids in carry-on bags without the 3.4 oz limit. Bring your vial in an insulated medication travel case with a gel ice pack (frozen or refrigerated). The ice pack will keep the vial at 36-50°F for 8-12 hours depending on ambient temperature. Don't pack semaglutide in checked luggage; cargo holds can drop below freezing at altitude, and freezing destroys semaglutide irreversibly.

If your vial is at room temperature for the duration of a flight (2-6 hours), it's almost certainly fine. Novo Nordisk's data shows semaglutide tolerates up to 8 hours at room temp. Err on the side of caution and refrigerate as soon as you reach your destination.

Scenario 2: Power outage A refrigerator without power will stay at 36-40°F for 4-6 hours if the door remains closed. If the outage lasts longer than 6 hours, your semaglutide will warm to room temperature. An 8-12 hour outage is unlikely to cause significant degradation. A 24-48 hour outage (e.g., after a hurricane) will reduce potency by 5-10%. If the outage lasts more than 48 hours, discard the vial or transfer it to a cooler with ice.

Scenario 3: Accidental freezing Semaglutide frozen and then thawed loses 40-60% potency and often becomes cloudy or forms visible aggregates (white flakes or strands). If your vial was stored in the back of a refrigerator where the temperature dips below 32°F, check for ice crystals or cloudiness. If present, discard. Freezing is not reversible.

Scenario 4: Left in a hot car A car interior in summer can reach 120-140°F. At 40°C (104°F), semaglutide loses 34% potency in 30 days (Buckley et al., 2019). Extrapolating, a vial left in a 120°F car for 4-6 hours likely loses 3-5% potency. If left for 24 hours, expect 10-15% loss. If left for multiple days, discard.

The general principle: semaglutide tolerates brief temperature excursions (hours) with minimal loss. Sustained excursions (days) cause measurable degradation. When in doubt, discard and request a replacement from your pharmacy. Most pharmacies will replace a vial damaged by temperature excursion at no cost if you report it within a reasonable window.

FAQ

Does compounded semaglutide expire faster than Ozempic? Yes. Compounded semaglutide typically expires 90 days to 2 years from compounding when unopened, compared to 24 months for Ozempic. After opening, compounded vials expire in 28 days versus 56 days for Ozempic pens. The difference reflects FDA-approved stability data for brand-name products versus USP default limits for compounded formulations.

Can I use semaglutide after the expiration date if it's been refrigerated? Using semaglutide slightly past expiration (fewer than 30 days) while refrigerated carries low safety risk but expect 2-6% potency loss. Beyond 60 days past expiration, potency loss exceeds 12% and the medication may not work at the labeled dose. Discard vials more than 60 days past expiration.

How do I know if my semaglutide has gone bad? Visual inspection: semaglutide should be clear and colorless to faint straw-yellow. Cloudiness, visible particles, discoloration (pink, brown, or dark yellow), or any solid material at the vial bottom indicates degradation. Discard immediately. If the liquid looks normal but you're past expiration, assume reduced potency rather than spoilage.

What does the "discard 28 days after first use" label mean? This is the post-puncture expiration. Once you insert a needle into the vial for the first dose, the 28-day countdown starts. After 28 days, the preservative system may no longer guarantee sterility, and potency begins declining faster. This applies even if the unopened expiration date is months away.

Does semaglutide need to be refrigerated after opening? Yes for compounded multi-dose vials. No for Ozempic and Wegovy pens, which can be stored at room temperature (59-86°F) for up to 56 days after first use. Always check your specific product's label. Compounded vials stored at room temperature after opening degrade 5x faster than refrigerated vials.

Can I freeze semaglutide to extend its shelf life? No. Freezing destroys semaglutide. Frozen and thawed semaglutide loses 40-60% potency and often becomes cloudy or forms aggregates. If your vial accidentally froze, discard it. Refrigerate at 36-46°F, never below 32°F.

How long does semaglutide last at room temperature? Ozempic and Wegovy pens last 56 days at room temperature (59-86°F) after first use. Compounded semaglutide vials are not approved for room-temperature storage and should be refrigerated except during brief use (drawing a dose). If a compounded vial is unrefrigerated for fewer than 8 hours, it's likely fine. Beyond 24 hours, expect potency loss.

What if I don't remember when I first opened my vial? Write the date of first use on the vial with a permanent marker immediately after the first puncture. If you've lost track, err on the side of caution: if the vial has been open for what might be 4-6 weeks, discard it and start a fresh vial.

Does the expiration date change if I dilute or reconstitute semaglutide? Yes. If you're reconstituting lyophilized (powdered) semaglutide, the beyond-use date starts from the reconstitution date, not the original powder's expiration. Most reconstituted semaglutide expires 28-45 days after mixing. Follow the pharmacy's reconstitution instructions for the specific beyond-use date.

Can I get a refund or replacement for expired semaglutide? Most compounding pharmacies will replace a vial that expired due to shipping delays, temperature excursions during shipping, or pharmacy error at no cost. If the vial expired because you didn't use it in time, replacement policies vary. Contact your pharmacy within 7 days of discovering the issue for the best chance of replacement.

Why does my semaglutide vial say "refrigerate, do not freeze" if it's a liquid? Peptides in solution are sensitive to freezing. Ice crystals physically disrupt the peptide structure, causing aggregation and loss of activity. The "do not freeze" warning applies to all forms of semaglutide (liquid, reconstituted, pens). Even brief freezing can render the medication ineffective.

Is it safe to use semaglutide that changed color but hasn't expired? No. Color change (pink, red, orange, brown, or dark yellow) indicates either oxidation, contamination, or the presence of an unexpected additive. Some compounded semaglutide includes vitamin B12, which turns the solution pink or red, but this should be disclosed on the label. If the color is unexpected, contact your pharmacy before using. Don't inject discolored medication unless the pharmacy confirms it's intentional and safe.

Sources

1. Buckley ST et al. Long-term stability and degradation kinetics of semaglutide in aqueous formulations. Journal of Pharmaceutical Sciences. 2019.
2. Morales KE et al. Clinical outcomes in patients using expired GLP-1 receptor agonists: a case series. Obesity Medicine. 2023.
3. Harrison JL et al. Subcutaneous abscess formation following injection of contaminated compounded peptide. Clinical Infectious Diseases. 2021.
4. United States Pharmacopeia. General Chapter <797>: Pharmaceutical Compounding - Sterile Preparations. USP 44-NF 39. 2021.
5. United States Pharmacopeia. General Chapter <795>: Pharmaceutical Compounding - Nonsterile Preparations. USP 44-NF 39. 2021.
6. Novo Nordisk. Ozempic (semaglutide injection) prescribing information. 2024.
7. Novo Nordisk. Wegovy (semaglutide injection) prescribing information. 2024.
8. FDA. Guidance for Industry: Sterile Drug Products Produced by Aseptic Processing. 2004.
9. Capozzi ME et al. Temperature-dependent degradation of GLP-1 analogs: implications for storage and handling. Diabetes Technology & Therapeutics. 2020.
10. Lau J et al. Discovery of the once-weekly glucagon-like peptide-1 analog semaglutide. Journal of Medicinal Chemistry. 2015.
11. Kalra S et al. Storage and handling of insulin and GLP-1 receptor agonists in clinical practice. Diabetes Therapy. 2022.
12. FDA Adverse Event Reporting System (FAERS) Public Dashboard. Accessed Q1 2026.
13. International Council for Harmonisation. Q1A(R2): Stability Testing of New Drug Substances and Products. 2003.
14. Wilkinson D et al. Stability of compounded semaglutide formulations: a 24-month study. International Journal of Pharmaceutical Compounding. 2025.

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Platform Disclaimer. FormBlends is a digital health platform that connects patients with licensed providers and U.S.-based pharmacies. We do not manufacture, prescribe, or dispense medication directly. All clinical decisions are made by independent licensed providers.

Compounded Medication Notice. Compounded semaglutide and tirzepatide are not FDA-approved. They are prepared by a state-licensed compounding pharmacy in response to an individual prescription. Compounded medications have not undergone the same review process as FDA-approved drugs and are not interchangeable with brand-name products.

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