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Thymosin Beta-4 Dosage Guide: Complete Guide

Thymosin Beta-4 (TB-500) dosage guide. Loading and maintenance phases, dosing by condition, frequency, and physician-guided protocols.

Reviewed by Form Blends Medical Team|Updated March 2026

Thymosin Beta-4 Dosage Guide: Complete Guide

Quick Answer: Thymosin Beta-4 dosage guide typically follows a two-phase approach: a loading phase of 2 to 4 weeks at higher doses, followed by a maintenance phase at reduced doses. Common loading doses range from 750 mcg to 2.5 mg injected subcutaneously twice per week. Maintenance doses are typically half the loading dose, administered once or twice weekly. Your physician will determine the exact protocol based on your condition, body weight, and treatment goals .

Standard Dosing Protocol

TB-4 Dosing Phases
PhaseDoseFrequencyDuration
Loading phase2-2.5 mg2x per week2-4 weeks
Maintenance phase1-1.5 mg1-2x per week4-8 weeks
Low-dose maintenance750 mcg1-2x per weekAs directed

The loading phase delivers higher doses to saturate tissue levels and initiate the repair cascade. Once healing is well underway, the maintenance phase sustains the benefit at lower doses .

Dosing by Condition

Acute Injury (Tendon, Ligament, Muscle)

  • Loading: 2-2.5 mg twice weekly for 4 weeks
  • Maintenance: 1-1.5 mg twice weekly for 4-6 weeks
  • Total course: 8-10 weeks

Chronic Tendinopathy

  • Loading: 2 mg twice weekly for 2-3 weeks
  • Maintenance: 1 mg twice weekly for 6-8 weeks
  • May require multiple treatment courses with breaks between

Wound Healing (Surgical, Skin)

  • Loading: 1.5-2 mg twice weekly for 2 weeks
  • Maintenance: 750 mcg-1 mg twice weekly until healed
  • Start 1-2 weeks before elective surgery when possible

Hair Growth

  • Loading: 2 mg twice weekly for 4 weeks
  • Maintenance: 1 mg once weekly for 8-12 weeks
  • Results take 2-3 months; longer treatment courses often needed

General Recovery and Wellness

  • 750 mcg-1 mg twice weekly for 4-8 weeks
  • Cycle off for 4-6 weeks, then reassess

Dosing Considerations

  • Body weight: Larger individuals may require doses at the higher end of the range. Some practitioners dose at approximately 20-30 mcg per kg of body weight
  • Severity of injury: More severe or chronic injuries typically warrant the loading phase approach, while minor injuries may respond to maintenance-level doses from the start
  • Combination therapy: When combined with BPC-157, some physicians reduce the TB-4 dose slightly since both peptides support healing through complementary mechanisms
  • Timing: TB-4 can be injected at any time of day. Some patients prefer morning dosing for convenience, while others inject at night to sleep through any initial fatigue

Injection Frequency and Timing

TB-4 has a relatively long half-life compared to some peptides, estimated at 2-3 hours in circulation but with tissue effects lasting much longer due to its intracellular mechanism of action. This is why twice-weekly dosing is sufficient for most protocols rather than daily injections .

Space injections evenly throughout the week (for example, Monday and Thursday, or Tuesday and Friday) for consistent tissue levels.

Frequently Asked Questions

Can I take more for faster healing?

Higher doses do not necessarily produce faster results and may increase side effects. Research shows a dose-response relationship up to a point, beyond which additional TB-4 provides diminishing returns. Follow your prescribed protocol.

What if I miss a dose?

Take the missed dose as soon as you remember, then resume your regular schedule. Do not double the next dose. TB-4's tissue effects are cumulative, so a single missed dose will not significantly impact your overall results.

How do I know if the dose is working?

Signs your dose is effective include reduced pain and swelling at injury sites (week 1-2), improved range of motion (week 2-4), and measurable healing progress on imaging or physical exam (week 4-8). If no improvement is seen after 4 weeks, discuss dose adjustment with your physician.

Get Your Personalized Protocol

At Form Blends, our physicians determine the optimal TB-4 dosing strategy for your specific condition and goals.

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Disclaimer: This article is for informational purposes only and does not constitute medical advice. Thymosin Beta-4 is not FDA-approved for any medical condition. Always consult with a licensed healthcare provider. Individual results may vary.

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